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rabbit anti vdac3  (Proteintech)


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    Structured Review

    Proteintech rabbit anti vdac3
    Rabbit Anti Vdac3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+vdac3+antibody/VDAC3+Antibody/pmc12953705-63-0-13
    Average 93 stars, based on 46 article reviews
    rabbit anti vdac3 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Modification:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Saline:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Staining:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Microscopy:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Mmp Assay:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Lysis:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Protease Inhibitor:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Blocking Assay:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Real-time Polymerase Chain Reaction:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    Isolation:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury
    Article Snippet: .. The materials used in this study were as follows: microglial mouse cell lines (catalog # CL-0493, Procell); high glucose Dulbecco’s modified Eagle’s medium (DMEM; catalog # C12430500BT, Gibco); 10% fetal bovine serum (FBS; catalog # 26010074, Gibco); DMEM without glucose (catalog # 11966025, Gibco); modular incubator chamber (catalog # MTC101, billups-rothenberg); single flowmeter (catalog # SFM3001, billups-rothenberg); dulbecco’s phosphate-buffered Saline (D-PBS, catalog # Lvn1023, livning); 0.4% trypan blue stain (catalog # C0040 solarbio); electron microscope fixing solution, (catalog #G1102, Servicebio); JC-1 MMP Assay (catalog # HY-K0601, medchemexpress); BBcellProbe M61 mPTP detection kit (catalog # BB-48122, BestBio); RIPA Lysis Buffer (catalog # C-1053, Applygen); Phenylmethanesulfonyl fluoride (PMSF; catalog # A1100, Applygen); Protease inhibitor cocktail (catalog # P1265-1, Applygen); NcmBlot blocking buffer (catalog # P30500, Ncmbio); rabbit anti-PINK1 antibody (catalog # 23274-1-AP, proteintech); rabbit anti-Parkin antibody (catalog # 32833, cell signaling technology); mouse anti-Parkin antibody (catalog # 4211, cell signaling technology); rabbit anti-VDAC3 antibody (catalog # 55260-1-AP, proteintech); rabbit anti-Bcl2 antibody (catalog # 26593-1-AP, proteintech); rabbit anti- Beclin-1 antibody (catalog # 3495 S, cell signaling technology); rabbit anti-Beta Actin antibody (catalog # 20536-1-AP, proteintech); rabbit anti-GAPDH antibody (catalog # 10494-1-AP, proteintech); rabbit anti-Cleaved Caspase 3 antibody (catalog # 9664, cell signaling technology); rabbit anti-caspase3 antibody (catalog # 14220, cell signaling technology); rabbit anti- SQSTM1/p62 antibody (catalog # 39749, cell signaling technology); rabbit anti-Cytochrom C antibody (catalog # 10993-1-AP, proteintech); rabbit anti-LC3B antibody (catalog # 43566, cell signaling technology); goat anti-rabbit HRP-conjugated IgG secondary antibody (catalog # ZB-2301, zsbio); Goat anti-Mouse IgG (H + L) Secondary Antibody, DyLightTM 488 (catalog # 35502, Thermo fisher scientific); Goat anti-Rabbit IgG (H + L) Secondary Antibody, DyLightTM 594(catalog # 35560, Thermo fisher scientific); HiScript III RT SuperMix for qPCR (+ gDNA wiper), (catalog # R323-01, Vazyme Biotech); FastPure Complex Cell/Tissue Total RNA Isolation Kit (catalog # RC113-C1, Vazyme Biotech); Taq Pro Universal SYBR qPCR Master Mix (catalog # Q712-03, Vazyme Biotech); QuickBlockTM Blocking Buffer for Immunol Staining (catalog # P0260, Beyotime). .. The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.

    other:

    Article Title: Hypothermia regulates mitophagy and apoptosis via PINK1/Parkin-VDAC 3 signaling pathway during oxygen-glucose deprivation/recovery injury.
    Article Snippet: The murine BV2 mouse microglial cells (catalog # CL-0493, Procell) were cultured in 10 cm dishes using high glucose DMEM supplemented with 10% FBS and cultured at 37°C in a 5% CO2 incubator.



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